Rabbit Anti-TUBB3, NT (TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III)
TUBB3, TUBB4, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III
Beta III tubulin is abundant in the central and peripheral nervous systems (CNS and PNS) where it is prominently expressed during fetal and postnatal development. As exemplified in cerebellar and sympathoadrenal neurogenesis, the distribution of beta III is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of this protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Indirect Flow Cytometry. Other applications have not been tested.
Recommended Dilution
Western Blot: 1:1000 Immunohistochemistry: 1:10-1:50 Indirect Flow Cytometry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 36-63 in the N-terminal region of human TUBB3. Species sequence homology: bovine, chicken, monkey, rat|
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human TUBB3 at ~55kD. Species crossreactivity: mouse
