043521
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
NP_057105.2Gene ID
51465Gene #
UBE2J1Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-UBE2J1, NT (UBE2J1, NCUBE1, Ubiquitin-conjugating enzyme E2 J1, Non-canonical ubiquitin-conjugating enzyme 1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E)
UBE2J1, NCUBE1, Ubiquitin-conjugating enzyme E2 J1, Non-canonical ubiquitin-conjugating enzyme 1, Yeast ubiquitin-conjugating enzyme UBC6 homolog E
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system.
Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
UBE2J1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBE2J1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Pattaro, C., et al. BMC Med. Genet. 11, 41 (2010) :|Olsen, J.V., et al. Cell 127(3):635-648(2006)|Oh, R.S., et al. J. Biol. Chem. 281(30):21480-21490(2006)|Wu, C.J., et al. EMBO J. 24(10):1886-1898(2005)|Mungall, A.J., et al. Nature 425(6960):805-811(2003)USBio References
No references available