Technical Data

043540
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NP_004779.2
Gene ID
9354
Gene #
UBE4A
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-UBE4A, ID (UBE4A, KIAA0126, Ubiquitin conjugation factor E4 A)
UBE4A, KIAA0126, Ubiquitin conjugation factor E4 A

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from the Central region of human UBE4A.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Shi, D., et al. Proc. Natl. Acad. Sci. U.S.A. 106(38):16275-16280(2009)|Sakiyama, T., et al. Inflamm. Bowel Dis. 14(3):310-317(2008)|Yang, Y., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 23(6):504-506(2007)|Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :|Spinette, S., et al. Cell Cycle 3(12):1638-1644(2004)
USBio References
No references available
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