Technical Data

043609
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Accession #
NP_006368.3;
Gene ID
10497
Gene #
UNC13B
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-UNC13B, NT (UNC13B, UNC13, Protein unc-13 homolog B, Munc13-2)
UNC13B, UNC13, Protein unc-13 homolog B, Munc13-2

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq].

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
UNC13B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 262-292 amino acids from the N-terminal region of human UNC13B.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :|Goldenberg, N.M., et al. Am. J. Physiol., Cell Physiol. 297 (4), C1053-C1058 (2009) :|Dimova, K., et al. Biochemistry 48(25):5908-5921(2009)|Tregouet, D.A., et al. Diabetes 57(10):2843-2850(2008)|Speight, P., et al. Traffic 6(10):858-865(2005)
USBio References
No references available
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