Technical Data

043652
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
O60763; NP_003706.1
Gene ID
8615
Gene #
USO1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-USO1, CT (USO1, VDP, General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, Vesicle-docking protein)
USO1, VDP, General vesicular transport factor p115, Protein USO1 homolog, Transcytosis-associated protein, Vesicle-docking protein

The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Transport from the ER to the cis/medial Golgi compartments requires the action of this gene product, GM130 and giantin in a sequential manner.

Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
USO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 931-960 amino acids from the C-terminal region of human USO1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Striegl, H., et al. PLoS ONE 5 (2), E8991 (2010) :|Merk, M., et al. J. Immunol. 182(11):6896-6906(2009)|Mukherjee, S., et al. J. Biol. Chem. 284(3):1709-1717(2009)|Striegl, H., et al. PLoS ONE 4 (2), E4656 (2009) :|Guo, Y., et al. Mol. Biol. Cell 19(7):2830-2843(2008)
USBio References
No references available
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