Technical Data

043655
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Mo
Accession #
NP_872294.2; O75317
Gene ID
219333
Gene #
USP12
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-USP12, ID (USP12, UBH1, USP12L1, Ubiquitin carboxyl-terminal hydrolase 12, Deubiquitinating enzyme 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12)
USP12, UBH1, USP12L1, Ubiquitin carboxyl-terminal hydrolase 12, Deubiquitinating enzyme 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12

USP12is a deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity. Not involved in deubiquitination of monoubiquitinated FANCD2.

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
USP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-251 amino acids from the Central region of human USP12.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Kee, Y., et al. J. Biol. Chem. 285(15):11252-11257(2010)|Asano, K., et al. Nat. Genet. 41(12):1325-1329(2009)|Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)|Dunham, A., et al. Nature 428(6982):522-528(2004)|Puente, X.S., et al. Nat. Rev. Genet. 4(7):544-558(2003)
USBio References
No references available
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