043709
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
PurifiedApplications
E IHC WBCrossreactivity
HuGene ID
7423Gene #
VEGFBShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-Vascular endothelial growth factor B, ID (VEGF2, VEGFB, VRF, Vascular endothelial growth factor B, VEGF-related factor)
VEGF2, VEGFB, VRF, Vascular endothelial growth factor B, VEGF-related factor
Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.
Applications
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Recommended Dilution
ELISA1:1,000 Western Blot1:50-100 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
VEGFB (VEGF2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117~147 amino acids from the Center region of human VEGF2.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Trompezinski, S., et al., Exp. Dermatol. 13(2):98-105 (2004).|Qi, J.H., et al., Nat. Med. 9(4):407-415 (2003).|Joukov, V., et al., J. Cell. Physiol. 173(2):211-215 (1997).|Olofsson, B., et al., J. Biol. Chem. 271(32):19310-19317 (1996).|Olofsson, B., et al., Proc. Natl. Acad. Sci. U.S.A. 93(6):2576-2581 (1996).USBio References
No references available