Technical Data

043720-PE
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
PE
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Accession #
NP_003361.1
Gene ID
7408
Gene #
VASP
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Rabbit Anti-VASP, CT (VASP, Vasodilator-stimulated phosphoprotein) (PE)
VASP, Vasodilator-stimulated phosphoprotein

Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.

Applications
Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Formalin-fixed, paraffin-embedded sections Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 267-296 in the C-terminal region of human VASP, UniProt Accession #P50552.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human VASP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Barragan, P., et al. Thromb. Haemost. 104(2):410-411(2010) 2. Dittrich, M., et al. Arterioscler. Thromb. Vasc. Biol. 30(4):843-850(2010) 3. Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010) 4. Osmancik, P., et al. Catheter Cardiovasc Interv 75(2):158-166(2010) 5. Siller-Matula, J.M., et al. J. Thromb. Haemost. 8(2):351-359(2010)
USBio References
No references available
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