Technical Data

043866
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_033542.2
Gene ID
22390
Gene #
Wee1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Wee1, ID (Wee1, Wee1-like protein kinase, Wee1A kinase)
Wee1, Wee1-like protein kinase, Wee1A kinase

Wee1 may act as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (By similarity).

Applications
Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 202-230 in the central terminal region of mouse Wee1. Species Sequence Homology: rat
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes human Wee1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Muller, M., et al. J. Cell. Sci. 123 (PT 2), 286-294 (2010). 2. Kim, M.J., et al. Oncol. Rep. 19(5):1323-1329(2008). 3. Tanaka, Y., et al. Biochem. Biophys. Res. Commun. 352(1):21-28(2007). 4. Tominaga, Y., et al. Int. J. Biol. Sci. 2(4):161-170(2006). 5. Park, C.E., et al. Cells Tissues Organs (Print) 177(4):221-228(2004)
USBio References
No references available
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