Technical Data

043881
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_060453
Gene ID
55062
Gene #
WIPI1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-WIPI1, NT (WIPI1, WIPI49, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49kD)
WIPI1, WIPI49, WD repeat domain phosphoinositide-interacting protein 1, Atg18 protein homolog, WD40 repeat protein interacting with phosphoinositides of 49kD

WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI1, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).

Applications
Suitable for use in Western Blot, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
WIPI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-59 amino acids from the N-terminal region of human WIPI1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Chasman, D.I., et al. PLoS Genet. 5 (11), E1000730 (2009) :|Proikas-Cezanne, T., et al. FEBS Lett. 581(18):3396-3404(2007)|Proikas-Cezanne, T., et al. Oncogene 23(58):9314-9325(2004)|Jeffries, T.R., et al. Mol. Biol. Cell 15(6):2652-2663(2004)
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net