043983
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
E FC IHC WBCrossreactivity
HuAccession #
NP_001070.2; NP_997402.1Gene ID
7535Gene #
ZAP70Shipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-ZAP70, ID (ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70kD zeta-chain associated protein, Syk-related tyrosine kinase)
ZAP70, SRK, Tyrosine-protein kinase ZAP-70, 70kD zeta-chain associated protein, Syk-related tyrosine kinase
This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry, ELISA
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-500 Indirect Flow Cytometry: 1:10-50 Immunohistochemistry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ZAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-304 amino acids from the Central region of human ZAP70.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
Zanotti, R., et al. Am. J. Hematol. 85(7):494-498(2010)|Lin, Y.P., et al. Mol. Immunol. 47 (11-12), 2022-2029 (2010) :|Liu, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(22):10166-10171(2010)|Kotaskova, J., et al. J Mol Diagn 12(3):328-334(2010)|Trojani, A., et al. Cancer Biomark 6(1):1-9(2010)USBio References
No references available