Technical Data

044055
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E FC WB
Crossreactivity
Hu
Accession #
NP_055610.1; NP_001165124.1
Gene ID
9839
Gene #
ZEB2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ZEB2, CT (ZEB2, KIAA0569, SIP1, ZFHX1B, ZFX1B, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b)
ZEB2, KIAA0569, SIP1, ZFHX1B, ZFX1B, Zinc finger E-box-binding homeobox 2, Smad-interacting protein 1, Zinc finger homeobox protein 1b

The protein encoded by this gene is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.

Applications
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications have not been tested.
Recommended Dilutions
Western Blot: 1:2000 Flow Cytometry (Indirect): 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1078-1105 in the C-terminal region of human ZEB2. Species Sequence Homology: mouse
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
Purity
Purified by Protein A and peptide affinity chromatography
Specificity
Recognizes human ZEB2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Kumar, P.A., et al. J. Biol. Chem. 285(41):31148-31156(2010). 2. Li, A., et al. Cancer Res. 70(13):5226-5237(2010). 3. Ohashi, S., et al. Cancer Res. 70(10):4174-4184(2010). 4. Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010). 5. Eriksson, N., et al. PLoS Genet. 6 (6), E1000993 (2010)
USBio References
No references available
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