Technical Data

044402
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Gene ID
7082
Gene #
TJP1
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-ZO1, CT (TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1)
TJP1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1

ZO1 encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Indirect Flow Cytometry
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:50-100 Immunohistochemistry: 1:50-100 Indirect Flow Cytometry: 1:10-50
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
ZO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1578~1607 amino acids from the C-terminal region of human ZO1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Specificity
Human

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Ohira, M., et al. Int. J. Mol. Med. 24(6):829-835(2009)|Hirakawa, H., et al. Int. J. Oncol. 35(6):1271-1276(2009)|Meerschaert, K., et al. Cell. Mol. Life Sci. 66(24):3951-3966(2009)|Kirschner, N., et al. Am. J. Pathol. 175(3):1095-1106(2009)
USBio References
No references available
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