Technical Data

044772
Clone Type
Monoclonal
Host
Human
Isotype
IgG2
Clone Number
G3 (recomb)
Grade
Highly Purified
Applications
E IC IP
Shipping Temp
Blue Ice
Storage Temp
-20°C
Human Anti-Enhanced Green Fluorescent Protein, Recombinant (EGFP)

anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Applications
Suitable for use in ELISA, Immunocytochemistry, and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution
Immunocytochemistry: 1:1000 Immunoprecipitation: 1:200 Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Enhanced Green Fluorescent Proteins (EGFP).
Form
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Purity
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Specificity
Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Product Reference: |●Fully in vitro selection of recombinant antibodies: S. Moutel, et al.; Biotech. J. 4, 38 (2009)|●
USBio References
No references available
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