Technical Data

044838
Clone Type
Monoclonal
Host
Human
Source
Human
Conjugate
ATTO 488
Isotype
IgG2
Clone Number
TA10
Grade
Highly Purified
Applications
IC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
+4°C/+4°C
Human Anti-Giantin (Recombinant) (ATTO 488) (Golgin Subfamily B Member 1, 372kD Golgi Complex-associated Protein, GCP372, Macrogolgin)
Golgin Subfamily B Member 1, 372 kDa Golgi Complex-associated Protein, GCP372, Macrogolgin

Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.

Applications
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human recombinant giantin.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).
Specificity
Recognizes human and mouse giantin.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Product Reference: |●Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003) |●Characterization of single chain antibody targets through yeast two hybrid: O. Vielemeyer, et al; BMC Biotechnol. 10, 59 (2010)|●
USBio References
No references available
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