Technical Data

044944
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rabbit Anti-IL-37 (Interleukin-1 Family Member 7, IL-1F7, FIL1 zeta, IL-1X, Interleukin-1 Homolog 4, IL-1H4, Interleukin-1 zeta, IL-1 zeta, IL-1RP1, IL1F7, FIL1Z, IL1H4, IL1RP1)
Interleukin-1 Family Member 7, IL-1F7, FIL1 zeta, IL-1X, Interleukin-1 Homolog 4, IL-1H4, Interleukin-1 zeta, IL-1 zeta, IL-1RP1, IL1F7, FIL1Z, IL1H4, IL1RP1

IL-37 (Interleukin-37) belongs to the IL-1 family. It binds to interleukin-18 receptor (IL-18R) but not to IL-1 receptor. It was shown to play an important role as a link between innate and adaptive immunity and it is suggested as a new player in the inflammatory and immune responses mediated by the IL-18/IL-18R axis. It was also shown to inhibit tumor growth.

Applications
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA (Direct and Indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween-20, 5% skim milk; suggested incubation time is 1 hour at RT Immunohistochemistry: 1:50 Immunoprecipitation: 1:500-1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human IL-37.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human IL-37. Detects a band of ~30kD by Western Blot.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net