Mouse Anti-NLRP3/NALP3 (NACHT-, LRR- and PYD-containing Protein 3, NLRP3, Cryopyrin, Cold Autoinflammatory Syndrome 1 Protein)
NACHT-, LRR- and PYD-containing Protein 3, NLRP3, Cryopyrin, Cold Autoinflammatory Syndrome 1 Protein
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1beta and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).
Applications
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and ChIP. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml Immunocytochemistry: See J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255 (2012) Immunohistochemistry: 1;200. See Nature 493, 67 (2012) Immunoprecipitation; In RIPA Buffer. See Mol. Cell 49, 331 (2012) ChIP: 2ug/DNA of 10e6 cells (see Nat. Immunol. 16, 859 (2015)) Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to mouse NLRP3/NALP3 (pyrin domain/aa1-93).
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Purity
≥95% (SDS-PAGE) from concentrated hybridoma tissue culture supernatant.
Specificity
Recognizes mouse NLRP3/NALP3. Species Crossreactivity: human.
