Technical Data

045101
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG1,k
Clone Number
16F11
Grade
Highly Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-Notch2 (Neurogenic Locus Notch Homolog Protein 2, Motch B)
Neurogenic Locus Notch Homolog Protein 2, Motch B

Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

Applications
Suitable for use in Flow Cytometry
Recommended Dilution
Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant mouse Notch2:Fc (AG-40B-0110).
Form
Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.
Purity
>95% (SDS-PAGE)
Specificity
Recognizes human and mouse endogenous Notch-2 receptor.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Product Reference: Dynamic Regulation of Notch 1 and Notch 2 Surface Expression during T Cell Development and Activation Revealed by Novel Monoclonal Antibodies: E. Fiorini, et al.; J. Immunol. 183, 7212 (2009)
USBio References
No references available
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