Guinea pig Anti-RBP4 (Retinol Binding Protein 4)
Retinol Binding Protein 4
Retinol binding protein 4 (RBP4; RBP) is a 21kD secreted protein, a member of the lipocalin family and is known as the primary transporter of retinol (vitamin A) to tissues. A recent report revealed RBP4 as an adipokine linking glucose transporter 4 (GLUT4) suppression in adipose tissue to insulin. Elevated human and mouse serum RBP4 levels are associated with insulin resistance and its severity, obesity, and certain components of metabolic syndrome. Furthermore, human serum RBP4 levels are closely related to renal function.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA (direct and indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human RBP4.
Form
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human RBP4. Detects a band of ~22-25kD by Western blot. Weakly cross-reacts with mouse and rat RBP4.
