Mouse Anti-RIG-I (RIG-1, Retinoic Acid-inducible Gene 1 Protein, DEAD-box Protein 58, Probable ATP-dependent RNA Helicase DDX58) (Biotin)
RIG-1, Retinoic Acid-inducible Gene 1 Protein, DEAD-box Protein 58, Probable ATP-dependent RNA Helicase DDX58
RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5'-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-beta (Cardif) in a CARD-CARD-dependent manner resulting in NF-kappaB and IRF3 activation.
Applications
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.
Recommended Dilution
Immunohistochemistry: paraffin sections Immunoprecipitation: 1:200 Western Blot: 1:1000 Optimal dilutions to be determined by researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa201-713 of human RIG-I.
Form
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography from concentrated hybridoma tissue culture supernatant.
Specificity
Recognizes human RIG-I. Species Crossreactivity: mouse
