Rabbit Anti-Sirtuin 1 (SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like Protein 1, SIR2L1)
SIRT1, NAD-dependent Deacetylase Sirtuin-1, SIR2-like Protein 1, SIR2L1
Sirtuin-1 is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Sirtuin-1 emerged as critical regulator of longevity and aging in several model organisms. Furthermore, a variety of age-associated diseases such as type 2 diabetes, obesity, osteoporosis, and Alzheimer's disease are associated with sirtuins. Sirtuin-1 has been suggested as potential biomarker for tumorigenesis.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA (direct and indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human Sirtuin 1.
Form
Supplied as a liquid in 0.2um-filtered PBS, pH 7.4. No preservative added.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Sirtuin 1. Detects a band of ~78kD by Western Blot.
