Mouse Anti-Sirtuin 2 (SIRT2, NAD-dependent Deacetylase Sirtuin-2, SIR2-like protein 2, SIR2L2)
SIRT2, NAD-dependent Deacetylase Sirtuin-2, SIR2-like protein 2, SIR2L2
Sirtuin-2 is a NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of a-tubulin. It is involved in the control of mitotic exit in the cell cycle. Furthermore, a variety of age-associated diseases such as type 2 diabetes, obesity, osteoporosis, and Alzheimer's disease are associated with sirtuins. Selective inhibition of Sirtuin-2 achieves neuroprotection and identifies Sirtuin-2 to be a potential therapeutic avenue in Huntington's and Parkinson's disease.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
ELISA (Direct and indirect): 1:2000-1:5000 Western Blot: 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at RT. Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to human Sirtuin 2.
Form
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Sirtuin 2. Detects a band of ~45kD by Western Blot.
