Technical Data

045377
Clone Type
Monoclonal
Host
Human
Isotype
IgG2
Clone Number
MB11
Grade
Affinity Purified
Applications
IC
Crossreactivity
Dr Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Human Anti-Tubulin-GTP (Recombinant)

Anti-Tubulin-GTP, monoclonal antibody (recombinant) (MB11) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Anti-Tubulin-GTP monoclonal antibody clone MB11 (recombinant) is composed of human variable regions (VH and VL) of immunoglobulin fused to the human lgG2 Fc domain.
Applications
Suitable for use in Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunocytochemistry: 1:10,000 Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length GTP-lambda-S loaded tubulin from porcine brain.
Form
Supplied as a liquid in PBS, 0.02% sodium azide, 10% glycerol.
Purity
Purified by Protein A affinity chromatography from HEK 293 cell culture supernatant.
Specificity
Recognizes tubulin-GTP. Species Crossreactivity: human, mouse, rat and drosophila.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Detection of GTP-Tubulin Conformation in Vivo Reveals a Role for GTP Remnants in Microtubule Rescues: A. Dimitrov, et al.; Science 322, 1353 (2008).|2. Preferential binding of a kinesin-1 motor to GTP-tubulin-rich microtubules underlies polarized vesicle transport: T. Nakata, et al.; J. Cell Biol. 194, 245 (2011).
USBio References
No references available
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