Rabbit Anti-NMDA NR1 Subunit, Splice Variant N1
GRIN1, N-methyl-D-aspartate Receptor Subunit NR1, NMD-R1, NMDAR1, Nmdar, NR1, GluN1, Glutamate [NMDA] receptor subunit zeta-1, Glutamate receptor ionotropic N methyl D aspartate 1, NMDAR
The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has been focused on the distribution and expression of these splice variants that may affect the functional properties and regulation of the NMDAR.
Applications
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: 1:1000-1:2000 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin, conjugated to KLH. Species sequence homology: canine, chicken, human, non-human primates and xenopus
Form
Supplied as a lyophilized powder. Reconstitute wtih 50ul sterile PBS.
Purity
Purified by affinity chromatography.
Specificity
Recognizes endogenous levels of the rat ~120kD NR1 subunit, N1 splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert. Species Crossreactivity: mouse
