Technical Data

045912
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a,k
Clone Number
SAM11
Grade
Affinity Purified
Applications
FC IHC WB
Crossreactivity
Hu Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-PAR-2 (PAR2, Proteinase-activated Receptor 2, Coagulation Factor II Receptor-like 1, G-protein Coupled Receptor 11, Thrombin Receptor-like 1)

The protein named Proteinase-activated receptor 2 (PAR2/PAR-2), or Coagulation factor II receptor-like 1, G-protein coupled receptor 11, Thrombin receptor-like-1 and encoded by the human gene F2RL1/GPR11/PAR2 is a receptor for trypsin and trypsin-like enzymes coupled to G proteins. Its function is mediated through the activation of several signaling pathways including phospholipase C (PLC), intracellular calcium, mitogen-activated protein kinase (MAPK), I-kappaB kinase/NF-kappaB and Rho. Also as part of the innate and adaptive immunity response, PAR2 acts as sensor for proteolytic enzymes generated during infection and works to promote inflammation and the inflammatory response. PAR2 is also involved in vascular and airway tone and in blood pressure regulation and many other G protein coupled receptor response pathways. PAR2 is a multi-pass membrane protein that is widely expressed in most tissues except brain or skeletal muscle.

Applications
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5ug/ml detected Par-2 in 10ug of NIH/3T3 cell lysate. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Linear peptide corresponding to the N-terminus of human Par-2.
Form
Supplied as a liquid in 0.1M Tris-Glycine pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human PAR-2. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net