045941
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG1Clone Number
1F11Grade
Affinity PurifiedApplications
FC IHC IP WBCrossreactivity
HuGene ID
5738Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-CD315
Prostaglandin F2 receptor negative regulator, PF2RI, SMAP-6, PTGFRN, FPRP, EWI-F
CD315, also known as prostaglandin F2 receptor negative regulator, is an approximately 135kD transmembrane glycoprotein, which associates with actin cytoskeleton, and with CD9 and CD81, but not with other tetraspanins. It seems to be involved in regulation of cell polarity and motility. CD315 is expressed mainly by keratinocytes, activated monocytes, and a subset of B cells, but it can be also used for distinguishing between strongly positive colon cancer and fibrosarcoma cells, and their negative normal cell counterparts.
Applications
Suitable for use in Immunoprecipitation, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 1-4ug/ml Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CD315.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Chambrion C, Le Naour F: The tetraspanins CD9 and CD81 regulate CD9P1-induced effects on cell migration. PLoS One 2010 Jun 21;5(6):e11219.|2. Charrin S, Manié S, Billard M, Ashman L, Gerlier D, Boucheix C, Rubinstein E: Multiple levels of interactions within the tetraspanin web. Biochem Biophys Res Commun. 2003 Apr 25;304(1):107-12.|3. André M, Chambrion C, Charrin S, Soave S, Chaker J, Boucheix C, Rubinstein E, Le Naour F: In situ chemical cross-linking on living cells reveals CD9P-1 cis-oligomer at cell surface. J Proteomics 2009 Nov 2;73(1):93-102.|4. Charrin S, Le Naour F, Oualid M, Billard M, Faure G, Hanash SM, Boucheix C, Rubinstein E: The major CD9 and CD81 molecular partner. Identification and characterization of the complexes, J Biol Chem. 2001 Apr 27;276(17):14329-37.USBio References
No references available