Technical Data

046093
Clone Type
Monoclonal
Host
Mouse
Source
E. coli
Isotype
IgG1
Clone Number
1D8D9
Grade
Affinity Purified
Applications
WB
Crossreactivity
Ec
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Mouse Anti-CRP (cAMP Receptor Protein, cAMP Regulatory Protein, Catabolite Gene Activator, Cap, Csm)

CRP (also known as cAMP receptor protein) is a regulatory protein in bacteria. CRP binding to cAMP leads to conformational changes in CRP resulting in a dramatic increase in its binding to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA and acts as a negative regulator of its own synthesis.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.25-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified protein
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Purity
Purified by affinity chromatography.
Specificity
Recognizes E. coli CRP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lauritsen I, et al. 2021. Nat Commun. 12:5880. |2. Ayhan D, et al. 2016. PLoS Biol. 14: 1002552. |3. Pryor R et al. 2019. Cell. 178(6):1299-1312 . |4. Seok JY, et al. 2021. Cell Reports. 36(8):109589.
USBio References
No references available
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