123327-HRP
Clone Type
PolyclonalHost
RabbitSource
HumanConjugate
HRPIsotype
IgGGrade
Affinity PurifiedApplications
WBCrossreactivity
HuAccession #
BC030957.1, AAH30957.1Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Rabbit Anti-ANK1 (Ankyrin-1, Ankyrin 1, ANK-1, Ankyrin-R, Erythrocyte Ankyrin, ANK) (HRP)
Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin actin cytoskeleton and therefore play a role in cell motility, proliferation and intercellular contact. Different isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains. Ankyrin 1, the prototype of this family, was first discovered in erythrocytes but has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated with hereditary spherocytosis. Different isoforms of ankyrin 1 have been described. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length human ANK1, aa1-155 (AAH30957.1).
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human ANK1.
