Annexin A2 (ANXA2), is an ~40kD member of the Annexin family of calcium-dependent phospholipid-binding proteins that are preferentially located on the cytosolic face of the plasma membrane. Phosphorylation of Annexin A2 at Tyr23 regulates its involvement in endosomal trafficking and actin cytoskeleton rearrangement. The Annexins consist of a unique amino terminal domain followed by a homologous C-terminal core domain containing the calcium-dependent phospholipid-binding sites. The C-terminal domain is comprised of four 60-70aa annexin repeats. Annexin A2 also functions as an autocrine factor to enhance osteoclast formation and bone resorption and is a major cellular substrate of the tyrosine kinase Src. Human Annexin A2 shares 97% identity with mouse and rat Annexin A2.

Suitable for use in Immunofluorescence, FLISA and Western Blot. Other applications not tested.

Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher.

MFTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETLILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Full length recombinant corresponding to aa1-339 from human ANXA2 (AAH09564) with GST tag. MW of the GST tag alone is 26kD.

Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purified by Protein A affinity chromatography.

Recognizes human ANXA2.