Technical Data

124008
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
2D4-2G11
Grade
Affinity Purified
Applications
E IP WB
Crossreactivity
Hu
Accession #
AAH09834
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-BRMS1 (Breast Cancer Metastasis-suppressor 1)

This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.

Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-246, from human BRMS1 (AAH09834) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human BRMS1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival. Cui RX, Liu N, He QM, Li WF, Huang BJ, Sun Y, Tang LL, Chen M, Jiang N, Chen L, Yun JP, Zeng J, Guo Y, Wang HY, Ma J.BMC Cancer. 2012 Aug 29;12(1):376. 2. Breast Cancer Metastasis Suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex. Kim B, Nam HJ, Pyo KE, Jang MJ, Kim IS, Kim D, Boo K, Lee SH, Yoon JB, Baek SH, Kim JH.Biochem Biophys Res Commun. 2011 Nov 9. 3. BRMS1 transcriptional repression correlates with CpG island methylation and advanced pathologic stage in non-small cell lung cancer. Nagji AS, Liu Y, Stelow EB, Stukenborg GJ, Jones DR.The Journal of Pathology (2010), DOI: 10.1002/path.2707. 4. Breast cancer metastasis suppressor 1 (BRMS1) suppresses metastasis and correlates with improved patient survival in non-small cell lung cancer. Smith PW, Liu Y, Siefert SA, Moskaluk CA, Petroni GR, Jones DR.Cancer Lett. 2009 Apr 18;276(2):196-203. Epub 2008 Dec 25. 5. Proteomics-Based Strategy To Delineate the Molecular Mechanisms of the Metastasis Suppressor Gene BRMS1. Rivera J, Megias D, Bravo J.J Proteome Res. 2007 Oct;6(10):4006-18. Epub 2007 Sep 14. 6. Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing HDAC1-mediated deacetylation of RelA/p65 and promoting apoptosis. Liu Y, Smith PW, Jones DR.Mol Cell Biol. 2006 Dec;26(23):8683-96. Epub 2006 Sep 25.
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net