Mouse Anti-CHD4 (Chromodomain-helicase-DNA-binding Protein 4, CHD-4, ATP-dependent Helicase CHD4, Mi-2 Autoantigen 218kD Protein, Mi2-beta)
The CHD family of proteins is characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. Patients with dermatomyositis develop antibodies against the nuclear antigen chromodomain helicase DNA binding protein 4. The protein exists in a complex containing histone deacetylase and nucleosome remodeling activities, suggesting a role for chromatin reorganization in cancer metastasis.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
ETEPKGAADVEKVEEKSAIDLTPIVVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNEERAATVTKKTYEIWH
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa1632-1730 from human CHD4 (NP_001264) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CHD4.
