Technical Data

125330
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a,k
Clone Number
2B11
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
BC011031, AAH11031
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-CRH (Corticoliberin, Corticotropin-releasing Factor, Corticotropin-releasing Hormone)

Corticotropin-releasing hormone (CRH) is a 41aa peptide derived from a 191aa preprohormone. CRH is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin deficiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, CRH may act as a trigger for parturition.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
SEEPPISLDLTFHLLREVLEMARAEQLAQQAHSNRKLMEIIGK*
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa154-196 from human CRH (AAH11031) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CRH.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. RelB/NF-?eB2 Regulates Corticotropin-Releasing Hormone in the Human Placenta. Wang B, Parobchak N, Rosen T.Mol Endocrinol. 2012 Jun 25.
USBio References
No references available
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