Technical Data

125394-HRP
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
HRP
Isotype
IgG1,k
Clone Number
1A9
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
BC021117, AAH21117
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
BSA Free
Mouse Anti-CSF1 (Macrophage Colony-stimulating Factor 1, CSF-1, M-CSF, MCSF, Lanimostim) (HRP)

Macrophage Colony Stimulating Factor (M-CSF) is produced by various cell types, including fibroblasts, microphages, and endothelial cells. M-CSF is involved in both the development and function of monocytes/microphages.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
EEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDK
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa33-132 from human CSF1 (AAH21117) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CSF1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pathway-Based Biomarker Search by High-Throughput Proteomics Profiling of Secretomes. Lawlor K, Nazarian A, Lacomis L, Tempst P, Villanueva J.J Proteome Res. 2009 Mar;8(3):1489-503.
USBio References
No references available
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