125980-APC
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
APCIsotype
IgG2b,kClone Number
3C11Grade
Affinity PurifiedApplications
FLISACrossreactivity
HuAccession #
BC063436, AAH63436Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
BSA Free
Mouse Anti-DOC2A (Double C2-like Domain-containing Protein alpha, Doc2, Doc2-alpha) (APC)
Doc2-alpha is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release. It may also be involved in dynein-dependent intracellular vesicle transport. The cDNA predicts a 400-amino acid protein with relative mass of 44,071 Da and 2 repeated C2-like domains in the C-terminal region. The C2 domain 1 is involved in binding calcium and phospholipids. It is predominantly expressed in the brain but also expressed in testis.
Applications
Suitable for use in FLISA. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA sequence
MRGRRGDRMTINIQEHMAINVCPGPIRPIRQISDYFPRGPGPEGGGGSGGEAPAHLVPLALAPPAALLGATTPEDGAEVDSYDSDDATALGTLEFDLLYDRASCTLHCSILRAKGLKPMDFNGLADPYVKLHLLPGACKANKLKTKTQRNTLNPVWNEDLTYSGITDDDITHKVLRIAVCDEDKLSHNEFIGEIRVPLRRLKPSQKKHFNICLERQVPLASPSSMSAALRGISCYLKELEQAEQGQGLLEERGRI
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-401 from DOC2A (AAH63436) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human DOC2A.
