Technical Data

126327
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b,k
Clone Number
3B4-1C5
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
BC034393, AAH34393
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Endothelin 2 (Endothelin-2, EDN2, ET2, ET-2, Preproendothelin-2, PPET2)

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MVSVPTTWCSVALALLVALHEGKGQAAATLEQPASSSHAQGTHLRLRRCSCSSWLDKECVYFCHLDIIWVNTPEQTAPYGLGNPPRRRRRSLPRRCQCSSARDPACATFCLRRPWTEAGAVPSRKSPADVFQTGKTGATTGELLQRLRDISTVKSLFAKRQQEAMREPRSTHSRWRKR
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-178 from human EDN2 (AAH34393) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human EDN2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Estrogen and progesterone regulate expression of the endothelins in the rhesus macaque endometrium. Keator CS, Mah K, Ohm L, Slayden OD.Hum Reprod. 2011 Apr 19. [Epub ahead of print]
USBio References
No references available
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