127043-Biotin
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
BiotinIsotype
IgG1,kClone Number
3G11-F9Grade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
BC032496, AAH32496Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Mouse Anti-FYN (Tyrosine-protein Kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like Kinase, SLK, p59-Fyn) (Biotin)
Fyn is a member of the Src family of protein tyrosine kinases. Fyn has been shown to be involved in T cell and B cell activation as well as keratinocyte differentiation. Fyn can regulate the activation of PI-3 kinase in CTLL-2 cells, a murine interleukin 2 responsive cell line. In T cells, Fyn associates with the T cell antigen receptor and Thy-1. A study of human T-cells revealed that Lck and Fyn had different intracellular locations, suggesting that these two members of the Src family regulate different aspects of T cell activation. The Src homology 3 (SH3) region of Fyn can bind the proline-rich domain of the Fas ligand. In addition, Raf-1 may form a complex with Fyn through its Src Homology 2 (SH2) domain and serine phosphorylation of Raf-1.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-482 from human FYN (AAH32496) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human FYN.
