127234
Clone Type
MonoclonalHost
MouseSource
HumanIsotype
IgG2a,kClone Number
2B8Grade
Affinity PurifiedApplications
E WBCrossreactivity
HuAccession #
BC041809, AAH41809Shipping Temp
Blue IceStorage Temp
-20°CMouse Anti-GCLM (Glutamate-cysteine Ligase Modifier Subunit, Gamma-glutamylcysteine Synthetase Regulatory Subunit, Gamma-ECS Regulatory Subunit, GCS Light Chain, Glutamate-cysteine Ligase Regulatory Subunit, GLCLR)
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSGASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-274 from human GCLM (AAH41809) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human GCLM.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway. Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, Macleod MC, Digiovanni J.Toxicol Appl Pharmacol. 2011 Jun 23. [Epub ahead of print] 2. Molecular and Functional Characterization of Gastrula Organizer Cells Derived from Human Embryonic Stem Cells. Sharon N, Mor I, Golan-Lev T, Fainsod A, Benvenisty N.Stem Cells. 2011 Feb 15. doi: 10.1002/stem.621. [Epub ahead of print] 3. Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease. Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ.Drug Metab Dispos. 2010 Dec;38(12):2293-301. Epub 2010 Aug 30. 4. Induction of Antioxidant Enzymes by Curcumin and Its Analogues in Human Islets: Implications in Transplantation. Balamurugan AN, Akhov L, Selvaraj G, Pugazhenthi S.Pancreas. 2009 May;38(4):454-60.USBio References
No references available