Technical Data

128235
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3H6
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NM_005533, NP_005524
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-IFI35 (FLJ21753, Interferon-induced 35kD Protein, IFP 35, Ifi-35, IFP35)

Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Sandwich ELISA: The detection limit is ~0.1ng/ml as a capture antibody Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
GVAQRLCQIGQFTVPLGGQQVPLRVSPYVNGEIQKAEIRSQPVPRSVLVLNIPDILDGPELHDVLEIHFQKPTRGGGEVEALTVVPQGQQGLAVFTSESG
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa189-288 from human IFI35 (NP_005524) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human IFI35.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Identification of Interferon-{alpha} induced genes associated with antiviral activity in Daudi cells, and characterization of IFIT3 as a novel antiviral gene. Schmeisser H, Mejido J, Balinsky CA, Morrow AN, Clark CR, Zhao T, Zoon KC.J Virol. 2010 Aug 4. [Epub ahead of print]
USBio References
No references available
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