Technical Data

129147
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b,k
Clone Number
3G1
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Hu
Accession #
NM_014916, NP_055731
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-LMTK2 (Lemur Tyrosine Kinase 2, LMR2, Apoptosis-associated Tyrosine Kinase 2, AATYK2, Brain-enriched Kinase, BREK, hBREK, CDK5/p35-regulated Kinase, CPRK, FLJ46659, KIAA1079, Kinase/Phosphatase/Inhibitor 2, KPI2, KPI-2, Serine/threonine-protein Kinase LMTK2, Serine/Threonine-protein Kinase KPI-2)

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families.

Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 0.7ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
EPAQTGVPQQVHPTEDEASSPWSVLNAELSSGDDFETQDDRPCTLASTGTNTNELLAYTNSALDKSLSSHSEGPKLKEPDIEGKYLGKLGVSGMLDLSED
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Partial recombinant corresponding to aa1181-1280 from LMTK2 (NP_055731) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human LMTK2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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