Technical Data

129503
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2a,k
Clone Number
6C2
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Accession #
BC009398, AAH09398
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-MCM7 (DNA Replication Licensing Factor MCM7, CDC47 Homolog, P1.1-MCM3, CDC47, MCM2)

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-389 from human MCM7 (AAH09398) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human MCM7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Etiological role of human papillomavirus infection for inverted papilloma of the bladder. Shigehara K, Sasagawa T, Doorbar J, Kawaguchi S, Kobori Y, Nakashima T, Shimamura M, Maeda Y, Miyagi T, Kitagawa Y, Kadono Y, Konaka H, Mizokami A, Koh E, Namiki M.J Med Virol. 2011 Feb;83(2):277-85. 2. Etiologic role of human papillomavirus infection in bladder carcinoma. Shigehara K, Sasagawa T, Kawaguchi S, Nakashima T, Shimamura M, Maeda Y, Konaka H, Mizokami A, Koh E, Namiki M.Cancer. 2010 Nov 29. [Epub ahead of print] 3. Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion. Lau KM, Chan QK, Pang JC, Li KK, Yeung WW, Chung NY, Lui PC, Tam YS, Li HM, Zhou L, Wang Y, Mao Y, Ng HK.Oncogene. 2010 Jul 26. [Epub ahead of print] 4. Validation of different replication markers for the detection of beta-cell proliferation in human pancreatic tissue. Kohler CU, Kreuter A, Rozynkowski MC, Rahmel T, Uhl W, Tannapfel A, Schmidt WE, Meier JJ.Regul Pept. 2010 Jan 14. [Epub ahead of print]
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net