Technical Data

130188
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
2G11-1C11
Grade
Affinity Purified
Applications
E IF WB
Crossreactivity
Hu
Accession #
BC001421, AAH01421
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-NDE1 (Nuclear Distribution Protein nudE Homolog 1, NudE, NUDE, FLJ20101, HOM-TES-87)

NDE1 is required for centrosome duplication. It is essential for the development of the cerebral cortex. It may regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex (By similarity). This protein is required for formation and function of the mitotic spindle.

Applications
Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution
Immunofluorescence: 10ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant corresponding to aa1-336 from human NDE1 (AAH01421) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human NDE1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. NDE1, NDEL1: Multimerisation, alternate splicing and DISC1 interaction. Bradshaw NJ, Christie S, Soares DC, Carlyle BC, Porteous DJ, Millar JK.Neurosci Lett. 2009 Jan 16;449(3):228-33. Epub 2008 Nov 6.
USBio References
No references available
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