Technical Data

130870-ML650
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™650
Isotype
IgG1,k
Clone Number
5F4
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Accession #
NM_003950
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Mouse Anti-PAR4 (Proteinase Activated Receptor 4, PAR-4, Coagulation Factor II Receptor-like 3, F2RL3, Thrombin Receptor-like 3) (MaxLight 650)

MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

Proteinase-Activated Receptor 4 (PAR4) is a member of the Proteinase-Activated Receptor subfamily. Along with other protease-activated receptors, PAR4 mediates the effects of the enzyme, thrombin which include activation of platelets, leukocytes, endothelial cells, and mesenchymal cells at sites of vascular injury. These cellular responses are triggered through proteolytic activation of the receptors by thrombin. It is believed that PAR3 functions as a cofactor for the cleavage and activation of PAR4. PAR4 expression has been documented in the periphery, primarily in blood platelets.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
GGTQTPSVYDESGSTGGGDDSTPSILPAPRGYPGQVCANDSDTLELPDSSRALLLGWVPTRLVPA
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa18-83 from F2RL3 (NP_003941) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human F2RL3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Polymorphism in the protease activated receptor-4 gene region associates with platelet activation and perioperative myocardial injury.|Muehlschlegel JD, Perry TE, Liu KY, Fox AA, Smith S, Lichtner P, Collard CD, Shernan SK, Hartwig JH, Body SC, Hoffmeister KM.American Journal of Hematology DOI: 10.1002/ ajh.22244
USBio References
No references available
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