131759-ML490
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™490Isotype
IgG1,kClone Number
3A10-2A11Grade
Affinity PurifiedApplications
FLISA WBCrossreactivity
HuAccession #
BC035857, AAH35857Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
Mouse Anti-PRDX6 (Peroxiredoxin-6, 1-Cys Peroxiredoxin, 1-Cys PRX, 24kD Protein, Acidic Calcium-independent Phospholipase A2, aiPLA2, Antioxidant Protein 2, Liver 2D Page Spot 40, Non-selenium Glutathione Peroxidase, NSGPx, Red Blood Cells Page Spot 12, AOP2, KIAA0106, MGC46173) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MPGGLLLGDVAPNFEANTTVGRIRFHDFLGDSWGILFSHPRDFTPVCTTELGRAAKLAPEFAKRNVKLIALSIDSVEDHLAWSKDINAYNCEEPTEKLPFPIIDDRNRELAILLGMLDPAEKDEKGMPVTARVVFVFGPDKKLKLSILYPATTGRNFDEILRVVISLQLTAEKRVATPVDWKDGDSVMVLPTIPEEEAKKLFPKGVFTKELPSGKKYLRYTPQP
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-224 from PRDX6 (AAH35857) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human PRDX6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Cellular and molecular large-scale features of fetal adipose tissue: is bovine perirenal adipose tissue brown?. Taga H, Chilliard Y, Meunier B, Chambon C, Picard B, Zingaretti MC, Cinti S, Bonnet M.J Cell Physiol. 2012 Apr;227(4):1688-700. doi: 10.1002/jcp.22893. 2. Functional analysis of beef tenderness. Guillemin N, Bonnet M, Jurie C, Picard B.J Proteomics. 2011 Aug 9. [Epub ahead of print] 3.Peroxiredoxin-6?XA potential protein marker for meat tenderness in bovine longissimus thoracis muscle. Jia X, Veiseth-Kent E, Grove H, Kuziora P, Aass L, Hildrum KI, Hollung K.J Anim Sci. 2009 Jul;87(7):2391-9. Epub 2009 Apr 9.USBio References
No references available