Technical Data

132219-APC
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
APC
Isotype
IgG2a,k
Clone Number
3B3
Grade
Affinity Purified
Applications
FLISA IP WB
Crossreactivity
Hu
Accession #
NM_177403, NP_796377
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
BSA Free
Mouse Anti-RAB7B (Ras-related Protein Rab-7b, RAB7) (APC)
MGC16212, MGC9726
Applications
Suitable for use in FLISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Sandwich FLISA: The detection limit is ~0.03ng/ml as a capture antibody. Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
DIWRGDVLAKIVPMEQSYPMVLLGNKIDLADRKVPQEVAQGWCREKDIPYFEVSAKNDINVVQAFEMLASRALSRYQSILENHLTESIKLSPDQSRSRCC*
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Partial recombinant protein corresponding to aa100-200 from human RAB7B (NP_796377) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human RAB7B.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Zlatic SA, Tornieri K, L'hernault SW, Faundez V.Mol Biol Cell. 2011 May;22(10):1699-715. Epub 2011 Mar 16. 2. Higashi S, Moore DJ, Yamamoto R, Minegishi M, Sato K, Togo T, Katsuse O, Uchikado H, Furukawa Y, Hino H, Kosaka K, Emson PC, Wada K, Dawson VL, Dawson TM, Arai H, Iseki E.J Neuropathol Exp Neurol. 2009 Sep;68(9):994-1005. 3.GIGYF2 is present in endosomal compartments in the mammalian brains and enhances IGF-1-induced ERK1/2 activation. Higashi S, Iseki E, Minegishi M, Togo T, Kabuta T, Wada K.J Neurochem. 2010 Jul 28. [Epub ahead of print]. 4. Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW.Mol Biol Cell. 2009 Feb;20(4):1223-1240. Epub 2008 Dec 24.
USBio References
No references available
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