133299-HRP
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
HRPIsotype
IgG1,kClone Number
3B7Grade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
BC006371, AAH06371Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Mouse Anti-SH3BGR (SH3 Domain-binding Glutamic Acid-rich Protein, SH3BGR Protein, 21-glutamic Acid-rich Protein, 21-GARP) (HRP)
Proline-rich peptide sequences have been shown to play important roles in protein-protein interactions that occur in signal transduction pathways. SH3 domain binding glutamic acid-rich protein (SH3BGR), also designated 21-glutamic acid-rich protein (21-GARP), is a 239aa protein differentially expressed in heart and skeletal muscle. Its proline-rich region contains the consensus sequence for an SH3-binding domain and its acidic C-terminal region contains a glutamic acid-rich domain which may assume a coiled-coil structure. SH3BGR may be part of a multimeric complex, as it contains 2 functional domains involved in protein-protein interactions.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
MPLLLLGETEPLKLERDCRSPVEPWAAASPDLALACLCHCQDLSSGAFPNRGVLGGVLFPTVEMVIKVFVATSSGSIAIRKKQQEVVGFLEANKIDFKELDIAGDEDNRRWMRENVPGEKKPQNGIPLPPQIFNEEQYCGDFDSFFSAKEENIIYSFLGLAPPPDSKGSEKAEEGGETEAQKEGSEDVGNLPEAQEKNEEEGETATEETEEIAMEGAEGEAEEEEETAEGEEPGEDEDS*
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-240 from human SH3BGR (AAH06371) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SH3BGR.
