133882-HRP
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
HRPIsotype
IgG2a,kClone Number
1A4Grade
Affinity PurifiedApplications
E IHC WBCrossreactivity
HuAccession #
BC002818, AAH02818Shipping Temp
Blue IceStorage Temp
-20°CNotes
Preservative Free
BSA Free
Mouse Anti-SSX2 (Synovial Sarcoma X Breakpoint 2, Cancer/testis Antigen 5.2, CT5.2, CT5.2a, Protein SSX2, SSX2A, SSX2B, Tumor Antigen HOM-MEL-40, MGC119055, MGC15364, MGC3884) (HRP)
Synovial sarcoma, X breakpoint 2, also known as SSX2, is a human gene. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX1, SSX2 nd SSX4 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. The SSX1 and SSX2 genes encode closely related proteins (81% identity) of 188aa that are rich in charged amino acids. The N-terminal portion of each SSX protein exhibits homology to the Kruppel-associated box (KRAB), a transcriptional repressor domain previously found nly in Kruppel-type zinc finger proteins.
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 1.5ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGRFSLSTSMGAVHGTPKTITHNRDPKGGNMPGPTDCVRENSW*
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full-length recombinant corresponding to aa1-224 from human SSX2 (AAH02818) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SSX2.