Technical Data

133882-ML550
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
MaxLight™550
Isotype
IgG2a,k
Clone Number
1A4
Grade
Affinity Purified
Applications
FLISA IHC WB
Crossreactivity
Hu
Accession #
BC002818, AAH02818
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Mouse Anti-SSX2 (Synovial Sarcoma X Breakpoint 2, Cancer/testis Antigen 5.2, CT5.2, CT5.2a, Protein SSX2, SSX2A, SSX2B, Tumor Antigen HOM-MEL-40, MGC119055, MGC15364, MGC3884) (MaxLight 550)

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Synovial sarcoma, X breakpoint 2, also known as SSX2, is a human gene. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. SSX1, SSX2 nd SSX4 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. The SSX1 and SSX2 genes encode closely related proteins (81% identity) of 188aa that are rich in charged amino acids. The N-terminal portion of each SSX protein exhibits homology to the Kruppel-associated box (KRAB), a transcriptional repressor domain previously found nly in Kruppel-type zinc finger proteins.
Applications
Suitable for use in FLISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (Formalin fixed paraffin embedded): 1.5ug/ml Optimal dilutions to be determined by the researcher.
AA Sequence
MNGDDAFARRPTVGAQIPEKIQKAFDDIAKYFSKEEWEKMKASEKIFYVYMKRKYEAMTKLGFKATLPPFMCNKRAEDFQGNDLDNDPNRGNQVERPQMTFGRLQGISPKIMPKKPAEEGNDSEEVPEASGPQNDGKELCPPGKPTTSEKIHERSGNREAQEKEERRGTAHRWSSQNTHNIGRFSLSTSMGAVHGTPKTITHNRDPKGGNMPGPTDCVRENSW*
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Full-length recombinant corresponding to aa1-224 from human SSX2 (AAH02818) with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human SSX2.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Expression and Immunotherapeutic Targeting of the SSX Family of Cancer-Testis Antigens in Prostate Cancer. Smith HA, Cronk RJ, Lang JM, McNeel DG.Cancer Res. 2011 Sep 7. [Epub ahead of print]
USBio References
No references available
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