Rabbit Anti-TRADD (Tumor Necrosis Factor Receptor Type 1-associated DEATH Domain Protein, TNFR1-associated DEATH Domain Protein, TNFRSF1A-associated Via Death Domain)
Tnfrsf1a-associated death domain (TRADD) is an adapter molecule for tnfrsf1a (tumor necrosis factor receptor superfamily 1a) that specifically associates with the domain of activated tnfrsf1a mediating its interaction with FADD. Overexpression of TRADD in human cells leads to two major TNF-related responses: apoptosis and activation of NF-kappa B. TNF in zebrafish was formerly known as ovarian tumor necrosis factor receptor (OTR). One study demonstrated that the extrinsic apoptosis pathway in zebrafish closely resembles its mammalian counterpart.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MAAGQNGHEEWVGSAYLFVESSLDKVVLSDAYAHPQQKVAVYRALQAALAESGGSPDVLQMLKIHRSDPQLIVQLRFCGRQPCGRFLRAYREGALRAALQRSLAAALAQHSVPLQLELRAGAERLDALLADEERCLSCILAQQPDRLRDEELAELEDALRNLKCGSGARGGDGEVASAPLQPPVPSLSEVKPPPPPPPAQTFLFQGQPVVNRPLSLKDQQTFARSVGLKWRKVGRSLQRGCRALRDPALDSLAYEYEREGLYEQAFQLLRRFVQAEGRRATLQRLVEALEENELTSLAEDLLGLTDPNGGLA
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length human TRADD, aa1-312 (NP_003780.1).
Form
Supplied as a liquid in PBS, pH 7.2.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human TRADD.
