Rabbit Anti-EPHB6 (EPH Receptor B6, MEP, HEP)
EPHB6 belongs to the ephrin receptor kinase family. EPHB6 does not have intrinsic tyrosine kinase activity because six invariant amino acids were have been substituted. Despite its lack of kinase activity, ligand binding results in trans-phosphorylation of EphB6 and the initiation of specific cytoplasmic signaling events.
Applications
Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 1:100-1:500 ELISA: 1:1000 Western Blot: 1:500-1:1000 Immunohistochemistry (Paraffin): 10ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human EPHB6 at C-Terminal.
Form
Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes human EPHB6. Species Crossreactivity: Mouse, rat.
