Rabbit Anti-Leukotriene B4 Receptor 1 (LTB4R1, LTB4-R 1, LTB4-R1, LTBR1, Leukotriene B4 Receptor, LTB4R, BLT, BLT1, BLTR, Chemoattractant Receptor-like 1, CMKRL1, G-protein Coupled Receptor 16, GPR16, P2Y Purinoceptor 7, P2RY7, P2Y7)
BLT1 is a Chemoattractant Receptor that binds the potent chemoattractant leukotriene B4 (LTB4) which is involved in defense against infection. Leukotriene B4 also activates the transcription factor PPAR alpha, resulting in the activation of genes that terminate inflammatory processes (Yokomizo et al., 1997). Although BLT1 shows homology to other P2Y receptors, it does not function as a purinoreceptor ( Yokomizo et al., 1997). Part of the coding regions of BLTR2 have been duplicated and are part of the 5' UTR noncoding regions of the clusters BLT1 and CIDE-B on chromosome 14.
Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:200 Immunohistochemistry (Parafin): 5ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa331-352 from human BLT1 (ALEPGPSESLTASSPLKLNELN).
Form
Supplied as a liquid in TBS pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human BLT1. Species Crossreactivity: Bovine. Does not react with mouse.
